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HPLC Columns

HPLC Columns
HPLC Columns
Product Code : 22
Brand Name : Neosphere
Product Description

Backed by a diligent team of professionals, we have been able to export and supply a excellent quality HPLC Columns. The provided columns are highly used for chromatography in various medical centers. Offered columns are manufactured by making use of optimum quality silica gel and pioneering technology at our vendor's sophisticated production unit. These columns are stringently tested on various quality parameters by our quality auditors in tandem with industry standards. Furthermore, these HPLC Columns are available at nominal prices.


  • Superior absorbency

  • Premium finish

  • Hydrophobic strength

  • Free from contamination


Neosphere is packed with ultra pure, spherical, completely porous silica gel, which is manufactured & packed under most stringently controlled conditions.

Our assurance for extra ordinary purity & are free from metallic contamination that could hinder optimum peak shape. Parameters controlled includes metallic impurities, hydrophobic strength, stearic selectivity, peak symmetry, silanopholic activity and ion exchange capacity.

This multiplicity of characteristics yields extremely reproducible chromatographic support providing reliable, contant batches, high performance separation columns.










Particle Sizes: 3 , 3.5‚¬, 5‚, 7‚, 10

Pore Size: 110 Ao

Surface Area: 320 m2/g

Chemical stability:
Neo Sphere phase are stable between pH 1.5 to 10 & high as 12 under certain conditions.
Fully end capped which gives high reproducibility & chemical stability.

Column Chart

Column Type

USP Code

Particle size

Dimension lengths (mm)

Dimension I.D. (mm)

Carbon loading %

C18 / RP18 / ODS 1-2


3, 5, 10

50, 100, 125, 150, 250, 300

4, 4.6


C8 / RP8 / OCTLY


3, 5, 10

50, 100, 125, 150, 250, 300

4, 4.6


C1 / RP1 / TMS


3, 5

50, 100, 125, 150, 250, 300

4, 4.6


C4 / RP4 / BUTY

3, 5, 10

50, 100, 125, 150, 250, 300

4, 4.6




3, 5, 10

50, 100, 125, 150, 250, 300

4, 4.6




3, 5, 10

50, 100, 125, 150, 250, 300

4, 4.6


Operational guidelines of Neosphere HPLC column.

All Neosphere columns are individually manufactured and tested to meet strict specifications criteria. Following instructions will enhance column performance and life.

  • Column connections: Afix the column as the direction of flow mentioned on column. For optimum performance reduce dead volume in the system by keeping tubing lengths between injector, column and detector as short as possible.

  • Equilibration: The storage solvent in a new column is mobile phase used to evaluate the column unless otherwise specified on the chromatogram. Never pass any material through the column that may precipitate in storage solvent. Ensure that the column is fully equilibrated before starting an analysis.

  • Performance testing: It is recommended that the performance of column should be tested on arrival.

  • HPLC solvent: Use only HPLC grade solvent and freshly prepared aqueous buffer solution.

  • Mobile phase pH: Recommended mobile phase pH of Neosphere column is between 1.5 - 9.5 this will maximize column life.

  • Column Washing: It is recommended to wash the reverse phase column (C1, C4, C8, C18, CN) with HPLC grade water containing 5% methanol / Acetonitrile.

  • Storage: Washout all water and buffer from bonded silica column and store it in organic solvents and keep it in a cool area.

  • Fitting: Avoid excessive tightening of column from mechanical shocks, droppings and banging.

  • Pressure & Temperature: Max operating pressure will be 6000 psi (-400 bar) and temperature not exceeding 80o C.

Keeping in mind the advantages we offers quality products, cost benefits, quality services, we looking forward for your esteemed order.

Room No 37, 2nd Floor, Nand Bhavan, 59 Babu Genu Road, Princess Street, Mumbai - 400002, Maharashtra, India
Mr. Bankim Unadkat (Proprietor)
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